Thiol activated cytolysin

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August undefined, 2024

Webxins. These toxins lyse or modulate the function of all cells containing cholesterol in their membranes: a property irreversibly inhibited by cholesterol. Some of the thiol-activated toxins are also antibody-binding proteins and can activate the classical complement pathway. A distinctive feature of the thiol-activated toxins is the formation of extremely … Webpfam01289 (PSSM ID: 426182): Conserved Protein Domain Family Thiol_cytolysin,

Cell, Vol. 89, 685–692, May 30, 1997, Copyright Structure of a ...

WebThis toxin excreted by a Gram-positive bacteria Streptococcus pyogenes, under the classification of Thiol-activated cytolysin or CDCs. In order for Streptolysin O to work effectively, it needs a significant amount of cholesterol to be present in … WebNov 18, 1996 · We present the first reported crystallization of a member of the thiol-activated family of protein toxins. Perfringolysin O, a virulence factor of Clostridium perfringens, has been crystallized in two different forms by the hanging drop vapor diffusion method. In one form the toxin crystallizes with PEG 20000 in the orthorhombic space … tacky and loud nyt crossword

Diversity of enterococcal bacteriocins and their grouping in a new ...

WebA distinctive feature of the thiol-activated toxins is the formation of extremely large arc- and ring-shaped structures in target membranes. These structures are believed to represent … WebJul 31, 1997 · Here, we present the first crystal structure of a thiol-activated cytolysin, perfringolysin O, a member of a large family of toxins that kill eukaryotic cells by punching holes in their membranes ... Macromolecules … WebJan 15, 2000 · Members of the thiol-activated family of cytolysins are involved in the mechanism of pathogenesis of a number of Gram-positive species. While they are pore-forming toxins, their major pathogenic effects may be more subtle than simple lysis of host cells, and may include interference with immune cell function and cytokine induction. tacky 80s dresses

Structure of a Cholesterol-Binding, Thiol-Activated …

WebDec 31, 2024 · Among the toxins produced by serotype C and D strains, botulinolysin (BLY), a thiol-activated hemolysin, is a member of the CDC family. In contrast to BoNT, which has been intensively studied because of its extraordinarily potent toxicity, the pathogenic role of BLY remains unclear. WebJan 15, 2000 · Members of the thiol-activated family of cytolysins are involved in the mechanism of pathogenesis of a number of Gram-positive species. While they are pore … tacky 90\\u0027s wedding dressesWebDec 1, 2016 · Botulinolysin (BLY) is a toxin produced by Clostridium botulinum that belongs to a group of thiol-activated hemolysins. In this study, a protein exhibiting hemolytic activity was purified from the culture supernatant of C. botulinum serotype D strain 4947. The purified protein displayed a single band by sodium dodecyl sulfate polyacrylamide gel … tacky 90s fashion

"WebJan 15, 2000 · The thiol-activated cytolysin (TACY) family is a prominent group of bacterial toxins, of which streptolysin O (SLO) is the prototype. Members of this group are elaborated by more than 20 species of Gram-positive bacteria [1] ( Table 1 ) and are intimately involved in the pathogenesis of infections by species such as Arcanobacterium pyogenes ... " - Thiol activated cytolysin

Thiol activated cytolysin

The family of thiol-activated, cholesterol-binding cytolysins

WebDec 17, 2010 · INY is a pore-forming toxin that is activated by reducing agents and inhibited by excess cholesterol. It is active across a pH range of 4.5 to 6.0 but is inactive at pH 7.4. At sublytic concentrations, INY activates p38 mitogen-activated protein kinase and allows entry of fluorescent phalloidin into the cytoplasm of epithelial cells.

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WebStreptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. WebJan 1, 2000 · The thiol-activated cytolysin (TACY) family is a prominent group of bacterial toxins, of which streptolysin O (SLO) is the prototype. Members of this group are elaborated by more than 20 species of Gram-positive bacteria [ 1 ] ( Table 1 ) and are intimately …

The thiol-activated Cholesterol-dependent Cytolysin (CDC) family (TC# 1.C.12) is a member of the MACPF superfamily. Cholesterol dependent cytolysins are a family of β-barrel pore-forming exotoxins that are secreted by gram-positive bacteria. CDCs are secreted as water-soluble monomers of 50-70 kDa, that when bound to the target cell, form a circular homo-oligomeric complex containing as many as 40 (or more) monomers. Through multiple conformational chan… WebThiol-activated peptides with GG leader peptide: Bacteriocins secreted by signal peptide pathway: Cystibiotics with one disulphide bridge but without YGNGVXC motif near N-terminus: ... Cytolysin: IIa: Pediocin-like or ‘ Listeria ’-active with YGNGVXC motif near N-terminus and GG leader peptide :

Webthiol-activated cytolysin. Pathway: map02024 : Quorum sensing: Brite: KEGG Orthology (KO) [BR:ko00001] 09140 Cellular Processes 09145 Cellular community - prokaryotes 02024 … WebThiol-Activated Cytolysin and a Model of Its Membrane Form Jamie Rossjohn,* Susanne C. Feil,* family is referred to as thiol-activated cytolysins be-William J. McKinstry,* Rodney K. Tweten,† cause biochemical modification of a cysteine residue and Michael W. Parker* causes toxin inactivation, although this residue is not

WebMay 30, 1997 · The mechanisms by which proteins gain entry into membranes is a fundamental problem in biology. Here, we present the first crystal structure of a thiol-activated cytolysin, perfringolysin O, a member of a large family of toxins that kill eukaryotic cells by punching holes in their membranes. The molecule adopts an unusually elongated …

WebJan 12, 2001 · Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form. Cell. 1997; 89: 685-692. Abstract; Full Text; Full Text PDF; PubMed; Scopus (402) Google Scholar) that may contain up to 50 individual monomers. The resulting transmembrane pore can be up to 30 nm in diameter (38. Sekiya K ; tacky adhesion—a preliminary studyWebDec 1, 2002 · The presence of the conserved undecapeptide cysteine residue either alone (His-PLO.C 492) or in a consensus sequence resulted in His-PLO molecules which were activated in the presence of reducing compounds, confirming the importance of this residue in the thiol-activated nature of many CDC toxins. tacky and loud crossword clueWebDec 1, 2001 · LLO, a pore-forming toxin of the thiol-activated cholesterol-dependent cytolysins (CDCs) family, is secreted as a soluble monomer that oligomerizes upon … tacky alcohol brandsWebMar 1, 2003 · Listeriolysin O (LLO, hly-encoded) is a major virulence factor secreted by the pathogen Listeria monocytogenes. The amino acid sequence of LLO shows a high degree of similarity with that of ivanolysin O (ILO), the cytolysin secreted by the ruminant pathogen Listeria ivanovii. Here, it was tested whether ILO could functionally replace LLO by … tacky accessoriesWebJan 26, 2013 · The toxin has a thiol-activated cytolysin which causes lysis of human platelets, lysosomes and a variety of subcellular membranes. The existing therapy seems to have challenged as available vaccines are not so effective and the bacteria developed resistance to many drugs. Computer aided approach is a novel platform to screen drug … tacky aestheticWebThiol-activated cytolysin Gene names Name pfo Synonyms pfoA, pfoR Ordered locus names CPE0163 Organism names Organism Clostridium perfringens (strain 13 / Type A) Taxonomic identifier 195102 NCBI Taxonomic lineage Bacteria > Firmicutes > Clostridia > Eubacteriales > Clostridiaceae > Clostridium Accessions Primary accession P0C2E9 tacky 90\u0027s wedding dressesWeb1.C.12 The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family. Cholesterol-binding 'sulfhydryl-activated' toxins bind to cholesterol containing animal cell membranes and can be reversibly inactivated by oxidation. The prototype of the family, perfringolysin O (PFO), can lyse cholesterol-containing membranes of eukaryotic host cells. tacky 80s wedding dress