Shrna lc3

Author: kwfq

August undefined, 2024

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shRNA慢病毒载体构建 - 哔哩哔哩

Splet06. mar. 2024 · In vitro, the increased LC3 puncta, the expression of LC3-II and Beclin-1, and the decreased expression of p62/SQSTM1 in SN-treated K562/ADR and K562 cells … SpletLC3（Atg8）是研究自噬必不可少的目标分子。慢病毒shRNA LC3-I/II主要用于感染或特别对传统转染试剂难于转染的细胞株、原代细胞、悬浮细胞和处于非分裂状态的细胞，以建立稳定下调/沉默LC3-I/II的细胞株。保存条件 -80℃保存，1年有效，-20℃保存， 1-2 个月内有效; 4℃保存， 1 周内有效。 tac force tf-934wp

A vector for co-expression of shRNA and shRNA-resistant cDNA.

Splet03. mar. 2024 · 病毒 shRNA 载体也可用于 RNAi 干扰，其带有抗生素标记的载体可以在细胞中持续抑制靶基因的表达，持续数星期甚至更久。相较于其它载体，病毒 shRNA 载体可 … Splet博尔森生物为您提供博尔森酶氨基酸试剂标物的型号、价格等信息，公司专业的售后服务团队，值得您的信赖。 http://www.sciben.com/Pc/Product/Details/b17e12c9-01ea-4fd2-b47f-04e02f7b6f42 tac force tf-428

Estrogen protects cartilage in rheumatoid arthritis JIR

Journal of Biological Regulators and Homeostatic Agents

SpletThe recruitment of LC3 to the phagosome is likely necessary for the degradation of a double membrane-wrapped phagosome. The fate of the midbody reveals how cells cope with released extracellular organelles. ... Next, we targeted the neutral sphingomyelinase 2 (nSMase2) pathway of EV synthesis using a shRNA to Smpd3, which encodes for this … Splet03. jul. 2014 · This permits ubiquitin-binding proteins (e.g., p62 and NBR1) to target impaired mitochondria to autophagosomes via Atg8/LC3II. Bcl-2 family proteins regulate mitochondrial outer membrane permeabilization during apoptosis and can also influence macroautophagy via interactions with Beclin-1. tac force tf-864Splet29. nov. 2024 · 我理解的那个说明书1：1的意思是，他lipo做了两管，dna做了一管，等一会混合的时候，把dna各分一半倒进lipo里我觉得也可以直接一比一做，就125的opti加2.5 … tac force training

"Splet15. apr. 2012 · Abstract. Neuroblastoma is an extracranial solid tumor that mainly occurs in children. It is derived from embryonic neural crest cells of the peripheral sympathetic nervous system. The overall survival rate of malignant neuroblastoma patients is very poor despite multi-modal therapy including surgery, radiotherapy, and chemotherapy. Efficacy … " - Shrna lc3

Shrna lc3

PLK1 contributes to autophagy by regulating MYC stabilization in ...

Splet15. maj 2024 · 经验总结：细胞转染那些事儿. 细胞转染对初接触细胞实验的科研人员而言，是一道难过的坎儿，细胞转染率低的话，你珍贵的细胞可能就只能扔掉了。. 大家都知 … Splet21. jun. 2024 · For HEI-OC1 cells, all of the LC3-RFP fluorescent puncta were counted in each HEI-OC 1 cell, and over 50 GFP-shRNA/LC3-RFP double-positive cells were counted. …

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Splet提供-心肌缺血再灌注损伤介绍和实验设计文档免费下载，摘要:.毒载体和特异性干扰的shrna序列分别过表达和敲低xxx的表达，并检测转染效率。随后检测细胞中乳酸脱氢酶(ldh)和超氧化物歧化酶(sod)含量，ldh和sod含量可以反映心肌受损程度，发现敲低xxx能够是细胞中的ldh和sod含量正常水 SpletPredesigned shRNA Predesigned shRNA 1 Search Genes/Clones 2 Select Clones 3 Specifications 4 Review & Confirm Help [email protected] 1-800-234-5362 …

Splet10. nov. 2024 · As third reaction, covalent attachment of ATG8/LC3 proteins to adipose molecules, typically to a phosphatidylethanolamine (PE), ... pmCherry-ATG5 construct by Jae-Won Soh or pSUPER-shRNA ATG5(2) with Adi Kimchi. This work was supported by the Technological and Technological Research Council of Turkey (TUBITAK) 1001 grant … Splet15. jul. 2016 · Two shRNA targeting LC3B (V2LHS_226621 and V3LHS_408637) were selected for their capacity to inhibit LC3-I and LC3-II in Western blotting and were …

Splet小髮夾RNA（英語：short hairpin RNA，縮寫shRNA）是一種形成急轉彎（hairpin turn）結構的RNA序列，可以經由RNA干擾（RNAi）使基因表現沉默化。 shRNA可利用載體導入細胞當中，並藉由U6啟動子來確保shRNA的表現。另外，shRNA可經由切割轉變成為siRNA 參考文獻[編輯] McIntyre G, Fanning G. Design and cloning strategies for constructing shRNA … Splet10. mar. 2024 · The RFP-GFP-LC3 assay in living cells was used to determine the autolysosomal flux in situ and impact of CDCA and LPS treatments. Autolysosomal flux …

SpletThe various elements are shown to scale: TRE, tetracycline response element; PminCMV, minimal immediate early cytomegalovirus (CMV) promoter; FLAG-3C-MCS, multiple …

http://www.honorgene.com/product_list/carrier_library/126615.html tac fordSpletLife Technologies公司有个非常实用的在线shRNA设计软件，操作非常方便，而且设计出来的shRNA不再需要到NCBI上Blast，直接可以用的。首先打开网站：http://rnaidesigner.lifetechnologies.com/rnaiexpress/，在Target Design Options处选中shRNA，以Fli1为例，输入Accession number或Nucleotide sequence，其他条件不变： … tac force vestSpletA. 用50ul无血清培养基稀释质粒DNA，轻轻混匀。B. 取适量合适的质粒转染试剂在50ul无血清培养基中稀释，室温孵育5min。C. 将前两步所稀释的DNA和质粒转染试剂混合，轻轻 … tac form armySpletSaos-2 cells were infected with an adenovirus expressing GFP-LC3 then treated with 0.1µM BafilomycinA1 or DMSO for 12h. Cells were then fixed and assayed for the ... by infection with retroviruses expressing respectively a non-silencing shRNA (sequence = GATAAGGTGCTGCGTGGACTTCA) and or a shRNA to target p53 (sequence = … tac forksSplet06. mar. 2024 · shRNA：小发卡或短发卡RNA (a small hairpin RNA or short hairpin RNA，shRNA)是一段具有紧密发卡环 (tight hairpin turn)的RNA序列，常被用于RNA干扰沉默靶基因的表达。利用载体把shRNA导入细胞，载体中的U6启动子确保shRNA总是表达；shRNA的发卡结构可被细胞机制切割成siRNA，然后siRNA结合到RNA诱导沉默复合物 … tac force tf-705Splet24. nov. 2024 · 目的：利用慢病毒感染的方法构建稳定敲低Atg5基因的RAW 264.7巨噬细胞系，为研究巨噬细胞自噬和免疫炎症相关疾病及其发生机制提供底盘细胞。. 方法：构建 … tac form nhsSpletAs disclosed herein, β-arrestin1 and β-arrestin2 levels are highly elevated in brains of FTLD-tau patients suggesting that both β-arrestin1 and β-arrestin2 are elevated in the brains of patients with AD and FLTD. The current work also shows that when β-arrestin2 is overexpressed, tau levels become elevated. The data indicate that β-arrestin2 reduces … tac form pdf