Webdays. Proliferation was measured by determining the extent of CFSC dilution. Mouse CTLL2 cells were cultured for 48 hr at the indicated concentration of IL-2 or ANV419, proliferation was quantified by incubation with WST-1 for 4 hr and measurement of OD at 450 nm. K D IL-2Ra(CD25) K D IL-2Rb(CD122) ANV419 No binding 377 nM IL-2-Fc 11.7 nM 1490 nM Web0574-87157013; [email protected]; 浙江省宁波市镇海区庄市街道兴庄路9号; 创e慧谷42号楼B幢401室
怎么养细胞?看这一篇就够了! - 知乎
WebJun 28, 2024 · 1)用RPMI-1640培养液洗涤对数生长期(培养3天)的CTLL-2细胞两次,每次离心250×g 10分钟,洗去培养液中残存的IL-2。. 2)用台盼蓝染色法计数细胞并决定 … http://www.bluefcell.com/productinfo/1473946.html how to set valorant tracker to private
CTLL-2 Cell Line - an overview ScienceDirect Topics
WebNov 4, 2024 · Since CTLL2 cells require IL-2 supplemented media, we rested cells in media free of IL-2 for five hours to reduce pSTAT5 expression prior to being stimulated with AnnV-IL2 or IL-2. Phospho-flow cytometry analysis showed that AnnV-IL2 was able to induce comparable pSTAT5 expression as IL-2 and suggests that the fusion does not impede … WebMar 28, 2024 · CTLL-2 [CTLL2]细胞:小鼠T淋巴细胞. HeLa [Chang Liver]细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:上皮细胞样;细胞生长特性:贴壁;细胞背景资料:Chang liver细胞是于1954年从非恶性的人体组织中建立的一株细胞系,Chang liver细胞被广泛应用 ... WebMar 15, 1993 · Absence of interleukin 2 (IL-2) from IL-2-dependent cells, such as the cytotoxic T-cell line CTLL2, causes DNA fragmentation and programmed cell death (apoptosis). We found that, upon initiation, DNA degradation proceeds rapidly. IL-2-deprived CTLL2 cells can be rescued from apoptosis by the additio … nothwest hvac boone nc