WebJan 15, 2024 · CircRNAs contain microRNA (miRNA) binding sites and can function as miRNA sponges to regulate gene expression by removing the inhibitory effect of an miRNA on its target gene. Methods:We used the bioinformatics software TargetScan and miRanda to predict circRNA-miRNA and miRNAi-Mrna interactions. WebRNA pulldown, mass spectrometry, chromatin immunoprecipitation, RNA-binding protein immunoprecipitation, chromatin isolation by RNA purification, electrophoretic mobility shift assay, and luciferase reporter assays were used to explore the molecular sponge role of the circRNA (via miRNA binding), and the interaction between circRNA and …
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WebJul 20, 2024 · Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs … WebcircRNA pull-down技术在circRNA与miRNA间相互作用研究中的应用 2024年Journal of Experimental & Clinical Cancer Research杂志上发表的文章中,作者使用了MS2序列作 … smackdown svr 2006
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WebTrying to unveil the underlying molecular mechanism, pull-down p53 immunoprecipitation evidenced that miR23-5p specifically and directly binds p53 in the cell nuclei of … WebTrying to unveil the underlying molecular mechanism, pull-down p53 immunoprecipitation evidenced that miR23-5p specifically and directly binds p53 in the cell nuclei of cardiomyocytes . ... The miRNA and circRNA world is characterized by a high complex behavior. In this context, the direct physical binding between p53 miRNAs or circRNAs ... WebApr 28, 2024 · 目标circRNA的机制研究 a RIP-qPCR:挑选功能最为明显的1个circRNA做RIP-qPCR实验,检测circRNA是否与AGO2蛋白结合。 (AGO2是circRNA发挥海绵作用的指示蛋白) b RNA pull down:对上述circRNA进行RNA pull down实验,拉下来的RNA进行定量PCR检测,检测生信预测中与该circRNA结合的10-20疾病miRNA。 solebury apple orchard